Sandwich ELISA, HRP/TMB, 12×8-well detachable strips

- Method
- Sample type
Serum, EDTA plasma, citrate plasma, heparın plasma, urine, cell culture supernatant, human and non-human samples
- Sample volume
10 µl / well
- Assay time
30 min / 2 h / 1 h / 30 min
- Sensitivity
0.09 nmol/l (= 6.3 ng/ml)
- Standard range
0 – 12 nmol/l (= 0 – 836 ng/ml)
- Conversion factor
1 pmol/l = 69.7 pg/ml (MW: 69.7 kDa)
- Specificity
Endogenous and recombinant human soluble Neuropilin-1 (isoform 2 and 3).
- Precision
In-between (n=10): ≤ 10 % CV
Within-run (n=6): ≤ 11 % CV
- Cross-reactivity
Due to the high sequence homology between human NPR1 and NPR1 of other species there is potential cross-reactivity with pig and rhesus macaque NPR1 and NPR1 of other species.
Neuropilin-1 (NRP1) only. The amino acid sequence in the respective binding regions of the antibodies show no homology with NRP2. Thus, cross-reactivity to NRP2 is not expected.
- Validation Data
See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc